Retrospective application of transposon-directed insertion site sequencing to a library of signature-tagged mini-Tn5Km2 mutants of Escherichia coli O157:H7 screened in cattle

Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
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Publication
Contributors
Eckert S E, Dziva F, Chaudhuri R R, Langridge G C, Turner D J, Pickard D J, Maskell D J, Thomson N R, Stevens M P
Year
2011
Journal
Journal of Bacteriology
Volume
193
Issue
7
Pages
1771-1776
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