Development of reagents to study the turkey's immune response: cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13

The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and trachea. Recombinant turkey IL-10 was expressed and bioactivity demonstrated by inhibition of IFN-gamma synthesis from activated splenocytes. Chicken and turkey IL-10 cross-reacted in functional assays.
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Publication
Contributors
Powell F, Rothwell L, Clarkson M, Kaiser P
Year
2012
Journal
Veterinary Immunology and Immunopathology
Volume
147
Issue
1-2
Pages
97-103
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